Course for international guest/part time students

Faculty

Faculty of Science

Organization

TTK Department of Organic Chemistry

Code

masspectk20em

Title

Mass Spectrometry

Usual semester

Autumn

Published semester

2026/27/1

ECTS

4

Language

en

Learning outcomes

Students can interpret mass spectra of organic compounds, peptides and proteins. Students know how mass spectrometers, ion sources, and analyzers operate, and know their application to solve analytical problems. Students can apply tandem mass spectrometry for structural or analytical studies. a) Knowledge - Knows the principle of operation, types, areas of use of mass spectrometers, the most important practical methods. Can interpret mass spectra, has the knowledge needed to interpret the mass spectra of an organic compound, peptide or protein at a basic level. He knows the related techniques, their fields of application. He is familiar with tandem mass spectrometry methods. b) Abilities - She is able to quickly master the operation of the most common types of mass spectrometers used in chemical research, development and innovation, and to interpret the obtained results. Evaluates the data obtained with a mass spectrometer objectively and critically, recognizes unsupported results. He is aware of the method that can be used to solve a particular analytical or structural research task based on mass spectrometry. c) Attitude - Open, motivated and receptive to learning about new, modern and innovative methods, to their practical application, open to paradigm shifts in science and technology, to applying new, previously unknown possibilities. Strives to use professionally justified, optimal and effective methods. d) Autonomy and responsibility - He operates mass spectrometers and the related equipment and devices responsibly, and acts with care in his work. Independently plans and manages measurements, evaluates the results with professional responsibility.

Course content

History of mass spectrometry - General set-up and operation of a mass spectrometer - Terminology, definitions - Analyzers and analyzer combinations - Ionization methods - Tandem mass spectrometry - Hyphenated techniques - Ion mobility mass spectrometry

Assessment method

Students are required to pass a comprehensive examination. The object of a first written part of the exam is to demonstrate general competence in two major topics in mass spectrometry. Result of the written part is discussed on the second, oral part of the comprehensive exam. Oral exam is organized on the day of the first written part. The object of the oral exam is to demonstrate the ability to verbally explain concepts and to demonstrate the ability to interpret mass spectral data.

Bibliography

Buday László, Nyitray László, Perczel András: Ezerarcú fehérjék (Semmelweis Kiadó és Multimédia Stúdió Kft., 2018) Dinya Zoltán: Szerves tömegspektrometria (Debrecen, 2001) John Greaves and John Roboz: Mass Spectrometry for the Novice (CRC Press, 2013) Vékey K, Telekes A, Vertes A.: Medical applications of mass spectrometry (Elsevier, 2008) R. B. Cole: Electrospray and MALDI Mass Spectrometry – Fundamentals, Instrumentation, Practicalities, and Biological Applications (Wiley, 2010)

Programmes of the course