Course for international guest/part time students
- Faculty
- Faculty of Science
- Organization
- TTK Department of Genetics
- Code
- eukgengb22em
- Title
- Eukaryotic Gene Regulation L
- Usual semester
- Autumn
- Published semester
- 2026/27/1
- ECTS
- 3
- Language
- en
- Learning outcomes
- Competencies: Knowledge: Understands the different levels of eukaryotic gene regulation. Understands the transcriptional level of regulation (role of cis and trans elements) and knows major processes related to chromatin regulation. Has knowledge about the posttranscriptional level of regulation. Has knowledge about the translational level of regulation. Understands the posttranslational level of regulation. Ability: Able to apply the acquired knowledge to explain everyday phenomena. Has the ability to reason based on science. Able to express himself/herself professionally in the field of genetics both orally and in writing. Attitude: Requires integrating the acquired information in his/her biological knowledge. Committed and open to science-based knowledge and an integrative approach. Committed to acquiring new competencies and expanding his/her worldview. Seeks to express a responsible opinion in the field of genetics. Autonomy and responsibility: Able to think about basic issues of genetics on his/her own and can report on them. Able to independently review the literature on a given topic, to organize and present the collected information. Feels responsible for acquiring scientifically based knowledge. Expresses opinions on issues related to genetics responsibly in professional and non-professional circles.
- Course content
- The aim of the course is to provide an integrated presentation about the different mechanisms of eukaryotic gene regulation at the transcriptional, posttranscriptional, translational and posttranslational levels. The most important topics of the lecture: Levels of eukaryotic gene regulation. Transcriptional level and the principle of cis-trans regulation of gene expression. Cis elements: promoters, enhancers, insulators. Enhancers as key elements ensuring spatial and temporal specificity of expression. Trans factors. Reporter genes. Identifying DNA-protein interactions. Regulation at the chromatin level: euchromatin-heterochromatin, chromatin remodelling, nucleosomes, histone code, promoter methylation. Position effect variegation (PEV), barrier insulators. Genomic imprinting. Methylation levels in the genome during development. X chromosome inactivation. Regulatory archipelagos (mouse HoxD cluster). Chromosome conformation capture technologies (3C, 4C). Posttranscriptional regulation: alternative and differential splicing. Example: Drosophila sex determination. Translational regulation: examples from Drosophila embryogenesis. Regulation at the posttranslational level: proteins, protein-protein interactions; examples of posttranslational modifications of proteins. Vulval development in C. elegans: examples of eukaryotic gene regulatory processes.
- Assessment method
- k5 = exam mark (5) (1 failed, 5 excellent) The evaluation is based on an assessment of students’ knowledge based on individual performance. Colloquium during the examination period (oral exam).
- Bibliography
- Lecture handouts (pdf)